Washburn University Chemistry Club

Topic:Hyperspectral Fluorescence Imaging of Biological Systems

Speaker:Dr. David M. Haaland, Senior Scientist, Sandia National Laboratories
Adjunct Professor, University of New Mexico

Abstract:
We have developed a 3D hyperspectral confocal fluorescence microscope that can optically section live cells with submicron spatial resolution. For each voxel within the specimen, the microscope records the entire emission spectrum from 500 nm to 800 nm. When coupled with multivariate curve resolution (MCR), the new microscope can “discover,” quantify, and resolve multiple spatially and spectrally overlapped emission components, thereby greatly increasing the number of fluorescent labels that can be monitored simultaneously. (Read more…)

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